Composite

Part:BBa_K3369002:Design

Designed by: Qinglin Zeng   Group: iGEM20_Tsinghua   (2020-10-23)


B.subtilis Nitric Oxide synthase with C4-HSL report system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1070
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1070
    Illegal NheI site found at 18
    Illegal NheI site found at 41
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1070
    Illegal BglII site found at 6
    Illegal BglII site found at 1225
    Illegal BamHI site found at 312
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1070
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 787
    Illegal SapI.rc site found at 1320


Design Notes

This part has been assembled to pSB1C3 plasmid. Still, silent mutation is needed to be made to meet the needs of assembly compability.


Source

The report system derives from BBa_K1893003. The NOS gene is cloned from B.subtilis genome.

References